| 渗透压法对牛血清蛋白在NaCl水溶液中相互作用的研究 |
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| 引用本文： | 陈得军,卢雁,谢冠群,张锁江,卢锦梭.渗透压法对牛血清蛋白在NaCl水溶液中相互作用的研究[J].化学研究与应用,2004,16(3):327-329,332. |
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| 作者姓名： | 陈得军 卢雁 谢冠群 张锁江 卢锦梭 |
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| 作者单位： | 河南师范大学化学与环境科学学院,河南,新乡,453002;中国科学院过程工程研究所,北京,100080 |
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| 基金项目： | 国家自然科学基金资助项目 (2 9773 0 11)，河南省教育厅科学基金资助项目 (2 0 0 0 115 0 0 0 12 ) |
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| 摘 要： | 测量了牛血清蛋白不同pH值和不同离子强度Nacl水溶液中的渗透压，计算了渗透第二维里系数，按照Prausnitz提出的分子热学模型计算了蛋白质之间的静电排斥能、色散吸引能和离子排斥体积产生的吸引势能，并对这三种势能与溶液pH值和离子强度的变化关系进行讨论。
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| 关 键 词： | 牛血清蛋白 渗透压 渗透第二维里系数 相互作用 |
| 文章编号： | 1004-1656(2004)03-0327-04 |
Study on the interactions of bovine serum albumin in aqueous NaCl solution by osmotic |
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| CHEN De-jun,LU Yan.Study on the interactions of bovine serum albumin in aqueous NaCl solution by osmotic[J].Chemical Research and Application,2004,16(3):327-329,332. |
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| Authors: | CHEN De-jun LU Yan |
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| Institution: | CHEN De-jun~1,LU Yan~ |
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| Abstract: | Using improved osmometers similar to those described by Viker et al,we measured the osmotic pressure of aqueous bovine serum albumin(BSA)solutions at four pH values(4.5,4.8,5.4,7.4)and at four sodium chloride concentrations(0.15M,1M,3M,5M).The osmotic second virial coefficients for BSA were calculated from the osmotic-pressure data.According to the molecular thermodynamic model presented by Prausnitz,the electrostatic repulsion potential attractive dispersion potential and the ion-excluded-volume potential have been calculated.The dependences of the three potentials on solution pH and on ionic strength were also discussed. |
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| Keywords: | bovine serum albumin osmotic pressure osmotic second virial coefficient interactions |
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