| 反相高效液相色谱对DABTH—氨基酸的分离与鉴定 |
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| 引用本文： | 殷长传,黄伟达.反相高效液相色谱对DABTH—氨基酸的分离与鉴定[J].高等学校化学学报,1993,14(3):328-331. |
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| 作者姓名： | 殷长传 黄伟达 |
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| 作者单位： | 复旦大学生物化学系，复旦大学生物化学系，复旦大学生物化学系 上海 200433，上海 200433，上海 200433 |
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| 摘 要： | 应用反相高效液相色谱(RP-HPLC)技术,采用等度洗脱。对20种标准DABTH-氨基酸进行了分离与鉴定,灵敏度达到pmol水平;并以溶菌酶为模式蛋白,经手工DABITC/PITC双偶联方法对其N端部分序列进行Edman降解,其降解产物DABTH-氨基酸用RP-HPLC技术进行了鉴定,蛋白质微量序列分析的灵敏度达到nmol水平.
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| 关 键 词： | DABTH 氨基酸 高效液相色谱 |
Separation and Identification of DABTH-Amino Acids by Reverse-Phase High-Performance Liquid Chromatography |
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| YIN Chang-Chuan,HUANG Wei-Da,SUN Chong-Rong.Separation and Identification of DABTH-Amino Acids by Reverse-Phase High-Performance Liquid Chromatography[J].Chemical Research In Chinese Universities,1993,14(3):328-331. |
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| Authors: | YIN Chang-Chuan HUANG Wei-Da SUN Chong-Rong |
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| Abstract: | Twenty standard DABTH-amino acids were separated and identified on a reverse-phase high-performance liquid chromatography(RP-HPLC) column with isocratic elution at a sensitivity of picomoles. By using the manual DABTH/PITC double-coupling method, we have analyzed and identified the N-terminal amino acid sequence of chicken lysozyme. This technique has been verified to be valuable for protein microsequencing at a level of nanomoles. |
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| Keywords: | Protein manual microsequencing DABTH-amino acid HPLC |
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