Changes in the physicochemical properties of transferrin upon chemical derivatization with ceramides |
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| Authors: | M A Egea M L García M A Alsina C Mestres F Reig |
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| Institution: | (1) Present address: Physicochemical Unity, Faculty of Pharmacy, University of Barcelona, Avda. Diagonal s/n Pedralbes, 08028 Barcelona, Spain;(2) Peptide Laboratory (CSID-CID), Barcelona, Spain |
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| Abstract: | The interaction of native and derivatized transferrin with lecithin, cholesterol and their mixtures was studied using monomolecular layers of phospholipids. Moreover, the thermodynamic parameters corresponding to these interactions: the mixing excess free energy, interaction energy, and interaction parameter were also calculated.The ability of these two proteins to insert into monolayers was studied working at constant area.The formation of aggregates or secondary structures in solution was determined by the fluorescence and polarization values of ANS (anilinonaphthalene sulfonate), measured at different protein/ANS relationships.Abbreviations TF
Transferrin
- DTF
derivatized transferrin
- PC
lecithin
- CHOL
cholesterol
- CER
ceramides
- ANS
anilinonaphthalene sulfonate
- TNBS
trinitro benzenesulfonate method |
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| Keywords: | Ceramides liposomes compression isotherms surface activity transferrin |
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