香叶天竺葵叶非精油组分清除自由基、抗氧化作用

活性氧与许多疾病的发生和发展密切相关,与食品的氧化也息息相关。所以筛选天然抗氧化剂显得非常重要。因此,分别采用水、乙醇和乙酸乙酯提取法从提取精油后的残渣中提取香叶天竺葵叶非精油有效组分,并分别采用超氧阴离子自由基(O2-·),羟自由基(·OH),过氧亚硝基(ONOO-)和.OH氧化损伤DNA的四种化学发光体系和二苯代苦味肼基自由基(DPPH·)和脂质过氧化两种比色体系检测香叶天竺葵叶非精油组分清除多种自由基、活性氧的能力以及对DNA的保护作用。结果表明,香叶天竺葵叶非精油组分的3种不同提取物均能有效地清除O2-·、·OH、DPPH·和ONOO-,减轻或消除·OH对DNA的氧化损伤,抑制脂质过氧化,是一种有效的、多功能的天然抗氧化剂和自由基清除剂。由于非精油组分是从制取精油组分后的残渣中提取的,提取装置和工艺也非常简单,故本研究不仅为香叶天竺葵种植效益的提高和综合利用提供了可能,也为天然香料加工后废弃物的开发研究提供了资料,对经济环境保护和资源的再利用也有借鉴意义。目前国内外对芳香植物的研究与开发主要聚焦于精油组分,而研究表明,更多的抗氧化物质是存在于非精油组分中,由于非精油组分是从制取精油组分后的残渣中提取的,并且提取装置和工艺简单,是值得进一步开发利用的天然产物。

Study on Scavenging Free Radical and Antioxidation Effects of Nonessential Oil Extracts from Pelargonium Graveolens Leaves

Activate oxygen is not only closely relevant with many disease's occurrent' but develops also going hand in hand with food oxidation. Therefore sifting natural antioxidant appears very important. The radical scavenging and antioxidant activities of extracts are compared and evaluated from the components of nonessential oils, which are distilled respectively by water extraction, ethanol extraction,and ethyl acetate extraction. Using chemiluminescence systems of superoxide anion, free radical, hydroxyl radical,peroxynitrite and DNA oxidative damage are caused by hydroxyl radical and colorimetric systems of DPPH free radical and lipid pexoxidation. The experiments reveal that the three different extractions of the components of nonessential oils in pelargonium graveolens leaves can not only directly scavenge superoxide anion free radical,hydroxyl radical,DPPH free radical and peroxynitrite, but also has a better ability to obviously mitigate and delay DNA oxidative damage caused by hydroxyl radical and inhibit lipid pexoxidation, so the components of nonessential oils in pelargonium graveolens leaves are effective and multifunctional natural antioxidants and radical scavenger,as we all know that the nonessential oil components is extracted from waste material of essential oil components,at the same time the abstract device and the handicraft is very simple, so our research improved pelargonium graveolens's planting efficiency, and provided possibility to it's comprehensive utilization,and provided data for further research on waste materials of natural spice processes, thus it has important reference meaning to economic environment protection and resource recycle . And at present, research to fragrant plant focuses on essential oil components, both home and abroad research indicated that more antioxidation matter is existence in nonessential oil components, as we all know that the nonessential oil components is extracted from waste material of essential oil components, at the same time the abstract device and the handicraft is very simple, so pelargonium graveolens is a kind of natural plant that is worthy of further being exploited and utilized.