|
|||||
|
|
| 树脂吸附法纯化赤霉素 | |
| 引用本文： | 王瑞芳,杨佳玲,史作清,欧来良. 树脂吸附法纯化赤霉素[J]. 离子交换与吸附, 2007, 23(3): 252-258 |
| 作者姓名： | 王瑞芳 杨佳玲 史作清 欧来良 |
| 作者单位： | 集美大学,生物工程学院,厦门,361021;天津市产品质量监督检测技术研究院,天津,300384;南开大学生物活性材料教育部重点实验室,天津,300071 |
| 基金项目： | 南开大学校科研和教改项目 |
| 摘 要： | 对比了几种大孔吸附树脂对赤霉素发酵液的吸附、解吸性能,实验发现,自制R4树脂的动态饱和吸附容量高达58.38mg/g干树脂,自制R5树脂的动态饱和吸附容量高达96.46mg/g干树脂,R5树脂的吸附容量明显高于R4,这与树脂的比表面积一致.50%(VIV)甲醇水溶液具有较好的解吸性能,收率高达95%以上,经过树脂一步吸附-解吸,赤霉素的浓度可平均提高5倍以上,集中收集最高可提高15倍以上,这对于工业化生产中,赤霉素的进一步结晶析出具有重要意义. |
| 关 键 词： | 赤霉素酸 吸附 大孔吸附树脂 分离 |
| 文章编号： | 1001-5493（2007）03-0252-07 |
| 修稿时间： | 2006-08-23 |
SEPARATION OF GIBBERELLIC ACID (GA3) BY MACROPOROUS ADSORPTION RESIN |
|
| WANG Ruifang,YANG Jialing,SHI Zouqing,OU Lailiang. SEPARATION OF GIBBERELLIC ACID (GA3) BY MACROPOROUS ADSORPTION RESIN[J]. Ion Exchange and Adsorption, 2007, 23(3): 252-258 | |
| Authors: | WANG Ruifang YANG Jialing SHI Zouqing OU Lailiang |
| Affiliation: | 1. College of Bio-engineering, Jimei University, Xiamen 361021, China; 2. Key Laboratory of Bioactive Materials, ministry of Education Nankai University, Tianjin 300071, China Tianfin Product Quality Inspection Technology Research Institute, 300384 China |
| Abstract: | Adsorption effects of several macroporous adsorption resins for gibberellic acid(GA3) were evaluated.The dynamic adsorption capacity on resin R4 is 58.38 mg/g dry beads,96.46mg /g dry beads for resin R5 which is consistent with surface area.Methanol aqueous(50% V/V) solution has the good elution effect and yield of GA3 is above 95%.Concentration of GA3 could be increased 5 times averagely after an adsorption-elution cycle and it is important to the crystal of GA3 on industry process. |
| Keywords: | Gibberllic acid(GA3) Adsorption Macroporous adsorption resin Separation. |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |