Preparative isolation and purification of syringin and edgeworoside C from Edgeworthia chrysantha Lindl by high-speed counter-current chromatography |
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Authors: | Yan Jizhong Tong Shengqiang Chu Jianjun Sheng Liuqing Chen Gang |
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Institution: | College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310032, China. yanjizhong@hzcnc.com |
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Abstract: | The bioactive compound syringin along with edgeworoside C were separated from the n-butanol extract of the stems and barks of Edgeworthia chrysantha Lindl (E. papyrifera) by high-speed counter-current chromatography (HSCCC) while it was difficult to purify each compound by silica gel column chromatography. Syringin was isolated from this plant for the first time. The two-phase solvent system used was composed of ethyl acetate-ethanol-water at an optimized volume ratio of 15:1:15 (v/v/v). Preparative HSCCC yielded, from 110mg of the partially purified extract, 28mg of syringin and 45 mg edgeworoside C each at over 96% purity by high-performance liquid chromatography analysis. Their structures were identified by electron impact ionization MS, 1H NMR and 13C NMR. |
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Keywords: | Counter-current chromatography Preparative chromatography Plant materials Pharmaceutical analysis Syringin Edgeworoside C |
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