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| 无定形硒纳米粒子的交联酶模板法合成及其在生物传感器中的应用 | |
| 引用本文： | 张娟,张胜义,徐静娟,陈洪渊.无定形硒纳米粒子的交联酶模板法合成及其在生物传感器中的应用[J].无机化学学报,2006,22(3):431-434. |
| 作者姓名： | 张娟 张胜义 徐静娟 陈洪渊 |
| 作者单位： | 1. 南京大学化学系,生命分析化学重点实验室,南京,210093;中国疾病预防控制中心改水中心,北京,102200 2. 南京大学化学系,生命分析化学重点实验室,南京,210093;安徽大学化学化工学院,合肥,230039 3. 南京大学化学系,生命分析化学重点实验室,南京,210093 |
| 摘 要： | 本文以戊二醛交联的辣根过氧化物酶(HRP)为模板合成了均匀分散的无定形硒纳米粒子(粒径10~20 nm)，以所得合成产物为载体构建了HRP生物传感器。研究结果表明，无定形硒纳米粒子具有良好的生物相容性和吸附性，所得传感器灵敏度高，对测定底物的生物亲和性好。 |
| 关 键 词： | 硒纳米粒子 辣根过氧化物酶 生物传感器 |
| 文章编号： | 1001-4861(2006)03-0431-04 |
| 收稿时间： | 2005-08-22 |
| 修稿时间： | 2005-12-20 |
Amorphous Selenium Nanoparticles: Synthesis in Horseradish Peroxidase Cross-linked and Application in Biosensor |
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| ZHANG Juan,ZHANG Sheng-Yi,XU Jing-Juan and CHEN Hong-Yuan.Amorphous Selenium Nanoparticles: Synthesis in Horseradish Peroxidase Cross-linked and Application in Biosensor[J].Chinese Journal of Inorganic Chemistry,2006,22(3):431-434. | |
| Authors: | ZHANG Juan ZHANG Sheng-Yi XU Jing-Juan and CHEN Hong-Yuan |
| Institution: | Key Laboratory of Analytical Chemistry for Life Sciences, Department of Chemistry, Nanjing University, Nanjing 210093;National Centre for Rural Water Supply Technical Guidance, China CDC, Beijing 102200,Key Laboratory of Analytical Chemistry for Life Sciences, Department of Chemistry, Nanjing University, Nanjing 210093;Department of Chemistry, Anhui University, Hefei 230039,Key Laboratory of Analytical Chemistry for Life Sciences, Department of Chemistry, Nanjing University, Nanjing 210093 and Key Laboratory of Analytical Chemistry for Life Sciences, Department of Chemistry, Nanjing University, Nanjing 210093 |
| Abstract: | Well-distributed amorphous selenium nanoparticles (10~20 nm) were synthesized by using the horseradish peroxidase (HRP) cross-linked with glutaraldehyde as template. The selenium nanoparticles as-prepared have been used to construct HRP biosensor. The results show that the HRP biosensor as-constructed has high sensitivity and affinity for H2O2 due to the good adhesive ability and biocompatibility of selenium nanoparticles. |
| Keywords: | selenium nanoparticles horseradish peroxidase biosensor |
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