A practical high-throughput screening system for enantioselectivity by using FTIR spectroscopy |
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Authors: | Tielmann Patrick Boese Matthias Luft Martin Reetz Manfred T |
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Institution: | Max-Planck-Institut für Kohlenforschung Kaiser-Wilhelm-Platz 1, 45470 Mülheim/Ruhr, Germany. |
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Abstract: | For the first time FTIR spectroscopy has been applied to the measurement of enantiomeric purity. The underlying concept is based on the use of pseudo-enantiomers that are (13)C-labeled at appropriate positions. Upon applying Lambert-Beer's law in the determination of the concentrations of both enantiomers, the ee values are accessible, accuracy to within +/-5 % of the true values being possible. The application of a commercially available high-throughput FTIR system results in a slightly decreased accuracy (+/-7% for the ee values), but this allows a throughput of up to 10000 samples per day. The method is of interest in the area of combinatorial symmetric catalysis and directed evolution of enantioselective enzymes. |
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Keywords: | asymmetric catalysis combinatorial chemistry directed evolution high‐throughput screening IR spectroscopy |
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