2'-modified-2-thiothymidine oligonucleotides |
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| Authors: | Rajeev Kallanthottathil G Prakash Thazha P Manoharan Muthiah |
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| Institution: | Department of Medicinal Chemistry, Isis Pharmaceuticals, Inc., Carlsbad, California 92008, USA. |
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| Abstract: | reaction: see text] Oligonucleotides with novel modifications, 2'-O-2-(methoxy)ethyl]-2-thiothymidine and 2'-deoxy-2'-fluoro-2-thiothymidine, have been synthesized. These modified oligonucleotides exhibited very high thermal stability when hybridized with complementary RNA. 2-O-(2-Methoxy)ethyl-2-thiothymidine modified oligonucleotide phosphodiesters showed enhanced resistance toward nucleases (t(1/2) > 24 h), but 2'-deoxy-2'-fluoro-2-thiothymidine modified oligonucleotide phosphodiesters showed limited stability to nucleotytic degradation. |
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