Highly Sensitive Neurotransmitters Analysis at Platinum‐Ultramicroelectrodes Arrays |
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| Authors: | Fernando C Moraes Ivana Cesarino Dyovani Coelho Valber A Pedrosa Sergio A S Machado |
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| Institution: | 1. Instituto de Química de S?o Carlos, Universidade de S?o Paulo, S?o Carlos, SP, Brasil;2. Institute of Bioscience, Department of Chemistry and Biochemistry, UNESP, Botucatu, SP, Brazil |
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| Abstract: | An easy way to determine norepinephrine (NE) in biological fluid using a platinum ultramicroelectrode array (Pt‐UMEAs) is described. Issues related to UME electrode surface treatment and characterizations are also addressed. At optimized experimental conditions the dynamic concentration range was 1.0 to 10.0 µmol L?1 with a detection limit of 40.5 nmol L?1. The repeatability of current responses for injections of 5 µmol L?1 NE was evaluated to be 4.0 % (n=10). This approach obtained excellent sensitivity, a reliable calibration profile and stable electrochemical response for norepinephrine detection. The content of NE in urine samples without any preconcentration, purification, or pretreatment step, was successfully analyzed by the standard addition method using the Pt‐UMEAs. |
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| Keywords: | Platinum ultramicroelectrode array Norepinephrine Biological fluids |
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