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Impedimetric Aptasensor for Thrombin Recognition Based on CD Support |
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| Authors: | Gabriela Castillo Libuse Trnkova Radim Hrdy Tibor Hianik |
| Institution: | 1. Department of Nuclear Physics and Biophysics, Faculty of Mathematics, Physics and Informatics, Comenius University, Mlynska dolina F1, 842?48 Bratislava, Slovakia;2. Department of Chemistry, Faculty of Science, Masaryk University, Kotlarska 2, 611?37 Brno and Central European Institute of Technology, University of Technology, Technicka 3058/10, 616?00 Brno, Czech Republic;3. Department of Microelectronics, Faculty of Electrical Engineering and Communication, Brno University of Technology, Technicka 10, 616?00 Brno, Czech Republic |
| Abstract: | We report an aptamer biosensing array for thrombin detection by measuring the electrochemical impedance upon aptamer‐protein formation at the surface of CD‐trodes (GCDTs) in the presence of the redox couple Fe(CN)6]3?/4?. GCDTs are fabricated from recordable compact discs that contain a fine gold layer. The biosensor is constructed by self‐assembling of a thiol‐modified thrombin binding aptamer (TBA) onto a GCDT surface. The sensor reveals good ligand specificity, recognition in a wide range of thrombin concentrations from 20 nM to 1 µM with a limit of detection of 5 nM. |
| Keywords: | DNA aptamers Biosensors Human thrombin Electrochemical impedance spectroscopy Gold CD‐trodes |