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| 不同体系下根霉脂肪酶失活动力学模型研究 | |
| 引用本文： | 尹春华,谭天伟,刘江帆.不同体系下根霉脂肪酶失活动力学模型研究[J].分子催化,2009,23(1). |
| 作者姓名： | 尹春华 谭天伟 刘江帆 |
| 作者单位： | 1. 北京科技大学应用科学学院生物技术系,北京100083;北京化工大学生命科学与技术学院,北京100029 2. 北京化工大学生命科学与技术学院,北京,100029 3. 北京科技大学应用科学学院生物技术系,北京,100083 |
| 摘 要： | 对根霉脂肪酶储存稳定性(干燥状态)及在水中,甘油微量水中等不同体系的热失活机理进行了研究,并求出了动力学参数.结果表明该酶在不同的外部环境中热失活机理并不一致:干燥酶粉符合一级失活模型,酶水溶液和酶甘油微水溶液却符合两步串联失活机理,而且证实甘油对该脂肪酶的热稳定性有促进作用. |
| 关 键 词： | 脂肪酶 动力学 失活 |
Thermal Inactivation Kinetics of the Rhizopus sp. Lipase |
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| YIN Chun-hu,TAN Tian-wei,LIU Jiang-fan.Thermal Inactivation Kinetics of the Rhizopus sp. Lipase[J].Journal of Molecular Catalysis (China),2009,23(1). | |
| Authors: | YIN Chun-hu TAN Tian-wei LIU Jiang-fan |
| Institution: | 1.School of Applied Science;University of Science and Technology Beijing;Beijing 100083;China;2.College of Life Science and Technology;Beijing University of Chemical Technology;Beijing 100029;China |
| Abstract: | Thermal inactivation behaviors of the Rhizopus sp.Lipase in several systems were investigated.Thermal inactivation kinetics depend on the environment around the lipase: the dry powder form of the lipase follows a simple One-step two states while both the lipase in water and the lipase in glycerol containing minor amount of water(4 wt.%) properly fit Two-step series deactivation.Glycerol was confirmed to be in favor of the stabilization of the lipase.Furthermore,kinetics parameters of the inactivation models... |
| Keywords: | Lipase Kinetic Deactivation |
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