一种新的基于正丁胺等离子体聚合膜的免疫传感器抗体(抗原)固定化方法

等离子体聚合膜 ( Plasma- polymerized film)是由有机蒸气在辉光放电下聚合而成 ,这种高度交联的膜具有均匀、超薄、附着力强、化学稳定、机械性能良好、基质类型多样以及成膜有机物品种多样等优点 ,因此已引起了传感器工作者的兴趣 ,目前 ,所研制的传感器已用于有机气体的测定 1 ,2 ] .Karube小组报道了乙烯二胺等离子体聚合膜在免疫传感器方面的应用3,4] .但由于抗体直接共价键合于等离子体聚合膜上 ,无法洗脱 ,使等离子体聚合膜修饰的传感器不能再生 ,而不同批次沉积的等离子体聚合膜其交联度、活性基团含量等又难以一致 ,严重影响了…

A New Method for Antibody(Antigen) Immobilization Based on Plasma-Polymerized Film

Anovel method based on plasma-polymerized films(PPFs) is proposed for immobilization of antibodies(antigens). PPFs are prepared on the surfaces of base sensors by a ratio frequency plasma method with n-butyl amine as a plasma source, and the IR spectrum indicates the existence of amino group in the film. Apolyanion layer is then self-assembled on the film through strong electrostatic attraction. Subsequently, antibodies(antigens) are immobilized on the self-assembled layer through controlling the pHvalue of immobilizing solution. The immobilization of goat-anti-IgG antibody, as an example, on the surfaces of quartz crystal microbalance (QCM) is investigated. After depositing a n-butyl amine PPFon the surface of QCM, a polystyrenesulfonate (PSS) layer is self assembled on the PPFfrom a 2.0 mg/mLof PSSsolution in 0.05 mol/L HCl. The goat anti IgGantibody is then immobilized in antibody immobolizing solution(0.2 mg/mL goat-anti-IgGantibody, pH5.0). This immobilization shows a better frequency response than the covalent immobilization with glutaraldehyde. The QCM immunosensor can quantitatively be used to determine NHIgG in the range of 0.7～126μg·mL^-1, and shows a good reproducibility. Moreover, the immunosensor can be easily regenerated by using a pHshift.