Origin of Fluorescence Lifetimes in Human Serum Albumin. Studies on Native and Denatured Protein |
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Authors: | Megdouda Amiri Kristina Jankeje and Jihad René Albani |
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Institution: | 1.Laboratoire de Biophysique Moléculaire,Université des Sciences et Technologies de Lille,Villeneuve d’Ascq Cédex,France |
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Abstract: | Human serum albumin consists of a single polypeptide of 585 amino acid residues with 1 Trp residue. In the present work, we
measured fluorescence lifetimes of the protein in both native and denatured states. The results indicate that Trp emission
occurs with three lifetimes in both states. Lifetimes values and contribution to the global emission decay differ between
the two states. Data are interpreted as the results of an emission occurring from three substructures of the tryptophan formed
in the excited state. Two of these substructures are already present for the tryptophan free in solution. The third lifetime
is the result of the interaction between the tryptophan residue and surrounding microenvironment. The populations of these
substructures characterized by the pre-exponential parameters of the fluorescence lifetimes are dependent on the fluorophore
microenvironment and on the global protein structure. |
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