Determination of L-tryptophan-serum albumin binding by high-performance liquid chromatography |
| |
Authors: | S F Sun Florence Wong |
| |
Institution: | (1) Department of Chemistry, St. John's University, 11439 Jamaica, New York |
| |
Abstract: | Summary Two high-performance liquid chromatography (HPLC) techniques were developed for the determination of binding constants in
the interaction of serum albumin with L-tryptophan: internal calibration and external calibration. The results obtained were
compared with those obtained by the classical method of equilibrium dialysis and by gel filtration. While all the methods
are equally reliable, the internal and external calibration techniques seem to be superior in their simplicity, speed and
convenience. |
| |
Keywords: | Column liquid chromatography L-tryptophan-serum albumin binding Bovine serum albumin Human serum albumin Binding parameters |
本文献已被 SpringerLink 等数据库收录! |