The binding of ethidium bromide with DNA: interaction with single- and double-stranded structures |
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| Authors: | Vardevanyan P O Antonyan A P Parsadanyan M A Davtyan H G Karapetyan A T |
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| Institution: | Biophysics Department of the Biological, Faculty of Yerevan State University, 375025 Yerevan, Aleck Manoogian St. 1, Armenia. |
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| Abstract: | The pH-induced helix-coil transition of DNA and its complexes with EtBr is carried out at acidic pH in a wide interval of change of concentration ratio of EtBr/DNA. The binding isotherms of EtBr on double and single-stranded DNA at pH = 7.0 and pH = 3.0 (t = 25(o)C) are obtained by absorption and fluorimetric methods. Binding constants (K) and number of bases (n), corresponding to one binding site were determined. Non fluorescent "strong" complex with ds-DNA at pH = 7.0 and t = 25(o)C as well as "strong" and "weak" complexes with ss-DNA at pH = 3.0 and t = 25(o)C are revealed. |
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