修复前后Vero细胞调控草酸钙晶体生长的差异

采用扫描电子显微镜(SEM)和流式细胞仪等多种方法研究了降解大豆多糖(SPS)对损伤的非洲绿猴肾上皮细胞(Vero)的修复作用;研究了修复前后Vero调控草酸钙(CaOxa)晶体形成的差异。经H2O2氧化损伤的Vero在被SPS修复后,其细胞活力、细胞外SOD活性及细胞内线粒体膜电位均增加,细胞形态逐渐恢复到接近正常细胞。在诱导草酸钙(CaOxa)晶体生长过程中,修复细胞可以减少棱角尖锐的一水草酸钙(COM)晶体生成,诱导更多的二水草酸钙(COD)晶体。三种状态Vero诱导的晶体尺寸从小到大顺序为:正常细胞<修复细胞<损伤细胞。本文结果表明,降解大豆多糖可以修复受损伤的Vero细胞,降低肾结石形成的危险性,提示SPS有可能是一种潜在的绿色防石药物。

Differences on Mediation of Calcium Oxalate Crystal Growth by Vero Cells before and after Repaired

The degradated soybean polysaccharides (SPS) were used to repair the injured African green monkey renal epithelial (Vero) cells, which were oxidatively injured by hydrogen peroxide (H₂O₂), by means of scanning electron microscopy (SEM) and flow cytometry etc. The differences on mediation of calcium oxalate (CaOxa) crystal growth by Vero cells before and after repaired were investigated. All the cellular viability, the activity of extracellular SOD and the intracellular mitochondrial membrane potential were increased after the repair by SPS to Vero cells. The morphology of injured Vero cells could be returned to plump state gradually after repaired. The repaired cells could reduce the formation of calcium oxalate monohydrate (COM) crystals with sharp edges and angles and increase the percentage of calcium oxalate dihydrate (COD) crystals. The size of the inductive CaOxa crystals by the three typological cells were as follows: normal cells < repaired cells < injured cells. The results in this study indicated that SPS could repair the injured Vero cells, thus reducing the risk of stone formation, and SPS may be a potential green anti-stone drug.