茶多酚锰合成、表征及络合和诱导肿瘤细胞凋亡的研究

合成了茶多酚锰(TP-Mn)和茶多酚锗(TP-Ge), 并采用红外光谱法及液相色谱质谱联用技术表征TP-Mn的理化特性. 选用反向液相色谱(RP-HPLC)和电感藕合等离子体质谱(ICP-MS)技术研究牛血清白蛋白(BSA)络合TP-Mn的能力, 指出BSA可直接络合于TP-Mn. 采用MTT法研究TP和TP-Mn诱导Hela卵巢癌细胞的凋亡率. 结果表明, TP和TP-Mn均能诱导Hela卵巢癌细胞凋亡, 但TP-Mn诱导凋亡率约为TP的2倍. 比较TP-Ge和TP-Mn诱导Raji人B淋巴瘤细胞凋亡率发现, 两者诱导肿瘤细胞的凋亡率几乎相同, 均高达86%左右. 人血清白蛋白(HSB)可作为输送TP-Ge和TP-Mn的药物载体.

Synthesis, Characterization and Binding of Tea Polyphenol-Manganese and Apoptosis Rate of Induced Tumor Cells

Both tea polyphenol-manganese(TP-Mn) and germanium(TP-Ge) were synthesized with organic synthesis approaches.The characterizations of TP-Mn were clearly revealed via infrared spectrometry and HPLC-ESI-Q-TOF mass spectrometry,respectively.Both HPLC and ICP-MS methods were employed to study the capacities for bovine serum albumen(BSA) binding to TP-Mn,indicating these capacities feasibility.Apoptosis rates in Hela ovarian induced with TP and TP-Mn were studied by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenly tetrazolium bromide(MTT) method.The experimental results show that both TP and TP-Mn can induced the cancer cell apoptosis,but the apoptosis rate with TP-Mn showed two times higher than that with TP.As compared experiments,the same apoptosis rates,going with up to approximately 86%,in Raji human B lymphoma induced with both TP and TP-Mn were observed directly.Human serum albumen might be employed to be a pharmacy carrier for transferring TP-Mn and TP-Ge in vivo.