Insulin-Loaded SLN Prepared with the Emulsion Dilution Technique: In Vivo Tracking of Nanoparticles after Oral Administration to Rats |
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| Authors: | M Trotta M E Carlotti M Gallarate G P Zara E Muntoni |
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| Institution: | 1. Dipartimento di Scienza e Tecnologia del Farmaco , Università degli Studi di Torino , Torino, Italy;2. Dipartimento di Anatomia, Farmacologia e Medicina Legale , Università degli Studi di Torino , Torino, Italy |
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| Abstract: | Insulin-loaded solid lipid nanoparticles (SLN) were prepared according to a solvent dilution method from O/W emulsions using isovaleric acid as organic phase. Insulin was derivatized with fluorescein isothyocianate (FITC) obtaining a fluorescent marker to be used in in vivo experiments. FITC-insulin and native insulin–loaded SLN were quite similar with regard to their mean sizes and encapsulation efficiency. SLN intestinal uptake was then investigated administering FITC-insulin loaded SLN on healthy male Wistar rats. Significant drug accumulation within intestinal lymphatic system was recovered, but the immune system seems to play an important role in SLN degradation: further studies are necessary to improve the results on blood glucose level. |
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| Keywords: | FITC-insulin intestinal uptake SLN |
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