A study on the two binding sites of hexokinase on brain mitochondria |
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Authors: | Abolfazl Golestani Hassan Ramshini Mohsen Nemat-Gorgani |
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Affiliation: | (1) Department of Clinical Biochemistry, School of Medicine, Medical Sciences/University of Tehran, Tehran, 14155-6447, Iran;(2) Institute of Biochemistry and Biophysics, University of Tehran, Tehran, 13145-1384, Iran;(3) Stanford Genome Technology Center, Stanford University, Palo Alto, CA, USA |
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Abstract: | Background Type I hexokinase (HK-I) constitutes the predominant form of the enzyme in the brain, a major portion of which is associated with the outer mitochondrial membrane involving two sets of binding sites. In addition to the glucose-6-phosphate (G6P)-sensitive site (Type A), the enzyme is bound on a second set of sites (Type B) which are, while insensitive to G6P, totally releasable by use of high concentrations of chaotropic salts such as KSCN. Results obtained on release of HK-I from these "sites" suggested the possibility for the existence of distinct populations of the bound enzyme, differing in susceptibility to release by G6P. |
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