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Fluorescence-based immunoassay for human chorionic gonadotropin based on polyfluorene-coated silica nanoparticles and polyaniline-coated Fe3O4 nanoparticles |
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| Authors: | Chu Chengchao Li Long Li Shuai Li Meng Ge Shenguang Yu Jinghua Yan Mei Song Xianrang |
| Institution: | 1.Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, People’s Republic of China ;2.Shandong Provincial Key Laboratory of Preparation and Measurement of Building Materials, University of Jinan, Jinan, 250022, China ;3.Cancer Research Center, Shandong Tumor Hospital, Jinan, 250012, People’s Republic of China ; |
| Abstract: | We report on an ultrasensitive fluorescence immunoassay for human chorionic gonadotrophin antigen (hCG). It is based on the use of silica nanoparticles coated with a copolymer (prepared from a fluorene, a phenylenediamine, and divinylbenzene; PF@SiO2) that acts as a fluorescent label for the secondary monoclonal antibody to β-hCG antigen. In parallel, Fe3O4 nanoparticles were coated with polyaniline, and these magnetic particles (Fe3O4@PANI) served as a solid support for the primary monoclonal antibody to β-hCG antigen. The PF@SiO2 exhibited strong fluorescence and good dispersibility in water. A fluorescence sandwich immunoassay was developed that enables hCG concentrations to be determined in the 0.01–100 ng·mL−1 concentration range, with a detection limit of 3 pg·mL−1.  Fluorescence detection of prepared immune reagent nano-composites using the fluorescence cell |
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