| REGULATION OF NITRATE REDUCTASE ACTIVITY IN RICE |
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| 作者姓名： | 林振武 汤玉玮 |
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| 作者单位： | Shanghai Institute of Plant Physiology，Academia Sinica，Shanghai Institute of Plant Physiology，Academia Sinica |
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| 基金项目： | Project supported by the National Natural Science Foundation of China. |
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| 摘 要： | The properties of NR from rice varieties Yuanfengzao and Jingyin 127 are the same in molecular weight of native enzyme and subunit, optimum pH, K_m value for substrates NO_3~- and NADH, stability in vitro and immunological characteristics. The decay rate in vitro of the activity of the partially purified NR from Yuanfengzao is 20％ lower than that from Jingyin 127. The results from antiserum titration and rocket immunoelectrophoresis indicated that the amount of NR in Yuanfengzao is about 1.5 times more than that in Jingyin 127. Further study on in vivo incorporation of ~3H-labeled amino acids into NR revealed that the rate of NR synthesis in Yuanfengzao is obviously higher than that in Jingyin 127. In vitro translation of mRNA showed that the products of NR-mRNA per unit of total mRNA of Yuanfengzao is much more than that of Jingyin 127. It is concluded that, therefore, NR activity in rice is regulated through NR synthesis and NR-inhibitory protein(s) system, the former is further controlled at the lev
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REGULATION OF NITRATE REDUCTASE ACTIVITY IN RICE |
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| LIN ZHENWU TANG YUWEI.REGULATION OF NITRATE REDUCTASE ACTIVITY IN RICE[J].Science in China(Chemistry),1988(10). |
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| Authors: | LIN ZHENWU TANG YUWEI |
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| Abstract: | |
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| Keywords: | nitrate reductase nitrate reductase-mRNA rice regulation property |
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