用丙烯酸类手性配位体交换树脂拆分DL-氨基酸的研究

本文利用以铜络合的手性L-脯氨酸聚丙烯酸类交换树脂为柱色谱的固定相,对DL-苯丙氨酸、DL-苯丝氯酸、DL-酪氨酸、DL-色氨酸、DL-组氨酸等芳香氨基酸的拆分进行了研究。实验结果表明,除了组氨酸之外,其它氨基酸均能完全拆开,且D型先于L型流出柱子。通过测定L-Pro-PGMA-Cu(Ⅱ)的红外光谱,提出了它在配位体交换过程中的配位结构模型。

Study of Resolution of DL Amino Acids with Ligand Exchange Chiral Resins

In this paper, resolution of DL-phc,PL-Phser, DL-Tyr, DL-Trp, DL-His etc was studied by liquid chromatography using L-Pro grafted resins coordinated with copper(Ⅱ) as stationary phase. Except DL-His, another amino acids mentioned above could be completely resolved and Dforms were eluated in front of Lforms Through determination of IR spectra of L-Pro-PGMA-Cu (Ⅱ) resin, its possible coordinated model of structure of resin and mechanism of resolution were presented.