高效液相色谱法分离/蒸发光散射和紫外检测法测定天麻中天麻甙含量

天麻（Gastrodia elata Blume）系兰科多年生寄生植物，用于治疗头错、眩晕、肢体麻木等症，冯孝章和周俊等分离并鉴定出天麻的活性成分有天麻甙（对羟甲基苯β－D－吡喃葡萄糖式，亦称天麻素）、天麻甙元（对羟基苯甲醇）等，其中天麻甙为主要成分，一些药理实验也证实了这一点，在测定天麻甙含量的方法中，采用高效液相色谱法9HPLC）最多，正相PHLC和反相HPLC都可用于天麻及其占天麻甙的分离，-通常采用紫外检测法，检测波长在220nm或270nm处。

Determination of Gastrodin in Gastrodia elata Blume by HPLC with ELSD and DAD

A normal-phase high performance chromatographic method with evaporative light-scattering detector(ELSD) and diode array detector(DAD), using n-hexane/methanol/ethyl acetate(volume ratio 6∶3∶2) as the mobile phase, was established for the direct determination of active constituents-gastrodin(GA) in the ethanol extracts of Gastrodia elata Blume. An silica gel column Zorbax RX-SIL46 mm (i.d.)×25 cm, 5 μm] was employed. Regression equations revealed the linear relationships(r=0.998 and 0.999) between the peak areas of GA measured by ELSD and DAD and its concentration. The recoveries of GA measured by ELSD and DAD are 104.22% and 97.65%, respectively. The contents of GA measured by ELSD and DAD are 13.9 mg/g(2.79% RSD) and 12.1 mg/g(2.99% RSD) respectively in Gastrodia elata Blume. The detection limits of ELSD and DAD(S/N=3) are 3 mg/L and 1 mg/L respectively. This method is simple, selective and sensitive.