| Institution: | (1) Postgraduate Education and Research Program in Analytical Chemistry, Faculty of Science, Kasetsart University, Jatujak, Bangkok, 10900, Thailand;(2) GSF-Research Center for Environmental and Health, Institute of Ecological Chemistry, Ingolstaedter Landstrasse 1, 85758 Oberschleissheim, Germany |
| Abstract: | A confirmation method for the determination of 18 aromatic amines originating from azo dyes after reductive cleavage was developed. The method is based on the use of high-performance liquid chromatography/tandem mass spectrometry with atmospheric-pressure chemical ionization. For the identification of the analytes one precursor ion and two daughter ions (multi-reaction monitoring, MRM) were selected and the LC-MS/MS parameters optimized to obtain high sensitivity and selectivity. The linear ranges varied from 0.1–1 to 30–50  g mL–1 with correlation coefficients of 0.99 or better. The applicability of the method to determine o-tolidine (3,3 -dimethylbenzidine) and 3,3-dimethoxybenzidine in textiles following reductive cleavage of acid red 114, trypan blue, and Chicago sky blue 6B was demonstrated. |
