Stability improvement of immobilized Candida antarctica lipase B in an organic medium under microwave radiation |
| |
Authors: | Réjasse Barbara Lamare Sylvain Legoy Marie-Dominique Besson Thierry |
| |
Institution: | Laboratoire de Biotechnologies et Chimie Bioorganique, CNRS FRE-2766, UFR Sciences Fondamentales et Sciences pour l'Ingénieur, Batiment Marie Curie, Université de la Rochelle, F-17042, La Rochelle cedex 1, France. |
| |
Abstract: | The influence of microwave heating on the stability of immobilized Candida antarctica lipase B was studied at 100 degrees in an organic medium. The microwave radiation was carried out before enzymatic reaction (storage conditions) or during the enzymatic catalysis (use conditions). In both cases, enzymatic stability was higher under microwave heating than under conventional thermal heating, in strictly identical operating conditions. Furthermore, the gain of enzymatic stability under microwave heating appears to be higher in a more polar solvent, which interacts strongly with the microwave field. Our results suggest that microwave radiation has an effect, not related to temperature, on the process of enzymatic inactivation. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|