Electrodeposited Cu‐Au Alloy Nanoparticles for Uric Acid Electrochemical Biosensor with Quick Response |
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| Authors: | Hongfang Zhang Yaping He Jianbin Zheng |
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| Institution: | 1. Key Laboratory of Synthetic and Natural Functional Molecule Chemistry Ministry of Education, Department of Chemistry, Northwest University, Xi'an 710069, P. R. China;2. Institute of Analytical Science/Shaanxi Provincial Key Laboratory of Electroanalytical Chemistry, Northwest University, Xi'an 710069, P. R. China |
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| Abstract: | A uric acid (UA) electrochemical biosensor based on the Cu‐Au alloy nanoparticles (NPs) and uricase was developed. The electrodeposition technique of Cu‐Au alloy NPs was selected to be a convenient potentiostatic method at –0.8 V in a single solution containing both Au(III) and Cu2+. Cyclic voltammetry and scanning electron microscopy proved the successful deposition of Cu‐Au alloy NPs. EIS demonstrated the good conductivity of Cu‐Au alloy NPs. The enzyme was immobilized on the surface of Cu‐Au alloy NPs modified electrode by casting with chitosan solution. The ultimate biosensor showed linear amperometric response towards UA in the concentration range of 3.0 to 26.0 μM with a detection limit of 0.8 μM. The main feature of the biosensor was its short response time, which was attributed to the good conductivity of Cu‐Au alloy NPs. Furthermore, the biosensor could avoid the interference of ascorbic acid and oxygen. |
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| Keywords: | Biosensor Electrodeposition Alloy nanoparticles Uric acid |
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