Relaxation of the folding of globulin around heme of hemoglobin of Homo sapiens by the food-grade additive molecule chlorophyllin |
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Authors: | Md Selim Arpita Sengupta Sadhu and Kalyan K Mukherjea |
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Institution: | (1) Department of Chemistry, Jadavpur University, Kolkata, 700032, India; |
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Abstract: | Abstract Binding of chlorophyllin (Chln), a food-grade additive molecule with hemoglobin (Hb), has been studied by photophysical and
photochemical methods with a view to unravel the biochemical transport pathway of it. The binding affinity constant and binding
sites between Chln and Hb are determined and found to be 3.3 × 105 M−1 and 15 (on tryptophan basis), respectively. Fluorimetric quenching experiments entail that Chln is bound in the vicinity
of the tryptophan residue of Hb. Circular dichroism studies suggest that Chln induces a change in the α-helical content of
Hb. Chlorophyllin is bound in the vicinity of the tryptophan residue of hemoglobin, which has been confirmed from spectrofluorimetric
studies, when a quenching in the tryptophan fluorescence occurs because of the chlorophyllin-induced exposure of the tryptophan
residue to hydrophillic zone. The cyclic voltammetric studies indicate that the redox reaction of FeII of Hb is inhibited shielding of it by the Chln molecule. |
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