| 高效液相色谱-质谱法测定特定用途保健药品中的褪黑激素 |
| |
| 引用本文： | 陈文锐,陈永红,胡国昌.高效液相色谱-质谱法测定特定用途保健药品中的褪黑激素[J].色谱,1998,16(5):451-453. |
| |
| 作者姓名： | 陈文锐 陈永红 胡国昌 |
| |
| 作者单位： | 国家进口食品卫生监督检验中心 |
| |
| 摘 要： | 采用ＨＰＬＣ－ＭＳ联用法测定了具有改善睡眠等特定功能的保健药品中的褪黑激素。利用质谱谱库检索和二极管阵列检测光谱图鉴定“松果体素”药品中的褪黑激素，以甲醇－水（７０∶３０）为流动相，检测波长为２７５ｎｍ。用外标法进行定量分析，方法最低检测限为０．２ｎｇ。
|
| 关 键 词： | 保健药品 高效液相色谱－质谱联用法 褪黑激素 |
The Determination of Melatonin in Health Caring Medicine for Specific Purposes by High Performance Liquid Chromatography Mass Spectrometry |
| |
| Chen Wenrui,Chen Yonghong and Hu Guochang.The Determination of Melatonin in Health Caring Medicine for Specific Purposes by High Performance Liquid Chromatography Mass Spectrometry[J].Chinese Journal of Chromatography,1998,16(5):451-453. |
| |
| Authors: | Chen Wenrui Chen Yonghong and Hu Guochang |
| |
| Institution: | National Centre of Import Food Hygiene Supervision & Inspection of P. R. China, Guangzhou, 510405. |
| |
| Abstract: | A rapid and precise method for the determination of melatonin in health-caring medicine for specific purposes, based on high performance liquid chromatography-mass spectrometry, has been developed. The compound was identified using DAD UV spectrum and mass spectrum. Hypersil ODS column, 100 mm x 2.1 mm i.d., and mobile phase of methanol-water (70:30, V/V) were used for the assays. The quantitation was performed with external standard, and detection at 275 nm. The calibration curve of melatonin was linear in the range of 5-50 mg/L and the detection limit was ca. 0.2 ng. |
| |
| Keywords: | high performance liquid chromatography/mass spectrometry melatonin health caring medicine |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《色谱》浏览原始摘要信息 |
| 点击此处可从《色谱》下载免费的PDF全文 |
