Silk-Fiber Immobilized Lipase-Catalyzed Hydrolysis of Emulsified Sunflower Oil |
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Authors: | Sushovan Chatterjee Lepakshi Barbora Swaranjit Singh Cameotra Pinakeswar Mahanta and Pranab Goswami |
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Institution: | (1) Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, 781039, India;(2) Centre for Energy, Indian Institute of Technology Guwahati, Guwahati, 781039, India;(3) Institute of Microbial Technology (IMT), Chandigarh, 160036, India |
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Abstract: | Lipase was immobilized in silk fibers through glutaraldehyde cross-linking to a maximum loading of 59 U/g silk-fiber and the
immobilized lipase was utilized for the hydrolysis of sunflower oil (Helianthus annuus). The hydrolytic activity of the lipase, which was poor in biphasic oil in water system, was increased significantly when
the sunflower oil was emulsified in aqueous medium. The hydrolytic activities of the immobilized lipase were 48.73 ± 1.26 U,
36.11 ± 0.96 U, and nil when the substrate sunflower oil was used as emulsion created by a rhamnolipid biosurfactant, Triton
X100, and ultrasonication, respectively. Although the efficiency of the immobilized lipase was less than 12% than the corresponding
free lipase, the immobilized lipase could be reused for the biosurfactant-mediated hydrolysis of sunflower oil up to third
cycle of the reaction. The yield of the fatty acids in the second, third, and fourth cycles were 49.45%, 22.91%, and 5.09%,
respectively, of the yield obtained in the first cycle. |
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Keywords: | Sunflower oil Lipase Immobilization Fatty acid Biosurfactant Silk fiber |
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