| 一种新的PEG凝聚人血清白蛋白液相压电免疫传感器 |
| |
| 引用本文： | 谢丽鹃,吴朝阳.一种新的PEG凝聚人血清白蛋白液相压电免疫传感器[J].分析化学,1998,26(8):927-930. |
| |
| 作者姓名： | 谢丽鹃 吴朝阳 |
| |
| 作者单位： | 湖南大学化学化工学院!化学计量学与化学传感器技术研究所!长沙410082(谢丽鹃，吴朝阳，沈国励)，湖南大学化学化工学院!化学计量学与化学传感器技术研究所!长沙41(俞汝勤) |
| |
| 基金项目： | 国家教委博士点基金，湖南省自然科学基金资助项目 |
| |
| 摘 要： | 以ＰＥＧ为乳剂，建立了一种基于ＰＥＧ凝集的液相压电免疫分析法，并用于人血清白蛋白（Ａｌｂ）的测定。详细考察了ＰＥＧ浓度，抗体稀释度、ＰＥＧ分子量及反应温度等因素对频移的影响，采用终点法和初始速率法测得Ａｌｂ线性范围分别为４７．８３－１０３．１ｍｇ／Ｌ和４０．６２－１０１．２ｍｇ／Ｌ。对７个血清样本的分析，其结果与双缩脲法基本一致。用一简单的方法合晶振再生后可获得重视的结果。
|
| 关 键 词： | 人血清白蛋白 Alb 免疫 传感器 分析 PEG凝聚 |
A Novel Polyethylene Glycol Agglutination-based Liquid Piezoelectric Immunosensor of Human Serum Albumin |
| |
| Xie Lijuan,Wu Zhaoyang,Shen Guoil,Yu Ruqin.A Novel Polyethylene Glycol Agglutination-based Liquid Piezoelectric Immunosensor of Human Serum Albumin[J].Chinese Journal of Analytical Chemistry,1998,26(8):927-930. |
| |
| Authors: | Xie Lijuan Wu Zhaoyang Shen Guoil Yu Ruqin |
| |
| Abstract: | A liquid piezoelectric immunoassay, which is based on the agglutination of antigen and anti- body polyethylene glycol (PEG) complex by an imunoreaction, has been developed for the determination of human serum albumin (Alb). The effect of experimental conditions such as PEG concentration, the antibody dilution ratio, the molecular weight of PEG and the reaction temperature on the frequency response were investigated. The linear ranges for Alb concentration determined by the end-point method and the initial rate method were 47. 83 - 103. 1 mg/L and 40. 62 - 101. 2 mg/l, respectively. Analytical results of seven clinical specimens obtained using the developed method were in satisfactory agreement with those given by the biuret method. With a simple regeneration method devised, the crystal can be used repeat- edly with acceptable reproducibility. |
| |
| Keywords: | Polyethylene glycol agglutination human serum albumin liquid peizoelectric immunosensor |
| 本文献已被 CNKI 维普 等数据库收录！ |
