Determination of acetazolamide in human urine samples by reversed-phase high-performance liquid chromatography in the presence of xanthines. |
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| Authors: | R Herráez-Hernández P Campíns-Falcó A Sevillano-Cabeza |
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| Affiliation: | Departamento de Química Analítica, Facultad de Química, Universidad de Valencia, Burjassot, Spain. |
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| Abstract: | ![]()
A simple, rapid and selective high-performance liquid chromatographic assay for the determination of acetazolamide in urine samples is described. After extraction with ethyl acetate, the drug is chromatographed on an HP-Hypersil ODS-C18 column with a mobile phase of acetonitrile-phosphate buffer (pH 3) and ultraviolet detection at 275 nm. The efficiency of the extraction, the linearity and the reproducibility of the method permit the evaluation of acetazolamide urinary excretion a long time after its administration. |
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