Effects of Metallic Silver Particles on Resonance Energy Transfer Between Fluorophores Bound to DNA期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Effects of Metallic Silver Particles on Resonance Energy Transfer Between Fluorophores Bound to DNA

Authors:	Joseph R. Lakowicz Józef Kuśba Yibing Shen Joanna Malicka Sabato D'Auria Zygmunt Gryczynski Ignacy Gryczynski

Affiliation:	(1) Department of Biochemistry and Molecular Biology, University of Maryland Baltimore, Center for Fluorescence Spectroscopy, 725 W. Lombard St., Baltimore, Maryland, 21201;(2) Faculty of Applied Physics and Mathematics, Technical University of Gdask, ul. Narutowicza 11/12, 80-952 Gdask, Poland

Abstract:	We examined the effects of metallic silver island films on resonance energy transfer (RET) between a donor and acceptor bound to double helical DNA. The donor was 4',6-diamidino-2-phenylindole (DAPI) and the acceptor was propidium iodide (PI). Proximity of the labeled DNA to the silver particles resulted in a dramatic increase in RET as seen from the emission spectra and the donor decay times. Proximity to silver particles results in an increase of the Förster distance from 35 Å an to an apparent value of 166 Å. These results suggest a new type of DNA hybridization assays based on RET over distances much longer than the free-space Forster distance.

Keywords:	Radiative decay engineering fluorescence resonance energy transfer silver nanoparticles labeled DNA
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