功能化ZnS纳米荧光探针荧光猝灭法测定丙硫氧嘧啶

研究了丙硫氧嘧啶对L-半胱氨酸包覆的ZnS纳米粒子荧光强度的影响,发现在pH=6.5的KH2PO4-NaOH缓冲溶液中,丙硫氧嘧啶对功能化ZnS纳米粒子有荧光猝灭作用,据此建立了以功能化的ZnS纳米粒子为荧光探针测定药物制剂中丙硫氧嘧啶含量的荧光分析法.丙硫氧嘧啶浓度在4.00×10-6~4.00×10-4mol.L-1范围内与体系的荧光强度线性关系良好,相关系数r=0.998 7,检出限(S/N=3)为1.70×10-6mol.L-1,对浓度为4.00×10-5mol.L-1丙硫氧嘧啶标准溶液平行测定11次的相对标准偏差为1.49%.该法用于丙硫氧嘧啶片中丙硫氧嘧啶含量测定,结果令人满意.

Fluorescence quenching method for the determination of propylthiouracil with functionalized ZnS nanoparticles as fluorescence probe

The effect of propylthiouracil on the fluorescence intensity of L-cysteine-capped ZnS nanoparticles was investigated.It was found that the fluorescence intensity of the functionalized ZnS nanoparticles could be quenched by propylthiouracil in the KH2PO4-NaOH buffer solution at pH=6.5.Accordingly,a sensitive fluorimetric method for determining propylthiouracil in pharmaceutical samples with functionalized ZnS nanoparticles as fluorescence probe was established.This method was simple and easy to operate.Under optimum conditions,the fluorescence intensity of the system showed a good linear relationship with the concentration of propylthiouracil in the range of 4.00×10-6 to 4.00×10-4 mol·L-1,the correlation coefficient was 0.9987 and the detection limit（S/N=3）was 1.70×10-6 mol·L-1.The relative standard deviation of replicate eleven measurements were 1.49% for 4.00×10-5 mol·L-1 propylthiouracil.The system was used for the determination of propylthiouracil in tablets with satisfactory results.