Effect of Constant Glucose Feeding on the Production of Exopolysaccharides by <Emphasis Type="Italic">Tremella fuciformis</Emphasis> Spores |
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Authors: | H Zhu S J Sun |
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Institution: | (1) Center for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao, 266555, People’s Republic of China;(2) College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, People’s Republic of China |
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Abstract: | A fed-batch culture system with constant feeding (glucose 80 g L−1, 0.25 ml min−1) was used to study the influence of glucose on cell dry weight and exopolysaccharides production from submerged Tremella fuciformis spores in a 5-L stirred-tank bioreactor. The results showed that high levels of cell mass (9.80 g L−1) and exopolysaccharides production (3.12 g L−1) in fed-batch fermentation were obtained after 1 h of feeding, where the specific growth rate (μ) and exopolysaccharides yield on substrate consumed (YP/S) were 0.267 d−1 and 0.14 g g−1. Unlike batch fermentation, maximal cell mass and exopolysaccharides production merely reached 7.11 and 2.08 g L−1; the specific growth rate (μ) and exopolysaccharides yield on substrate consumed (YP/S) were 0.194 d−1 and 0.093 g g−1, respectively. It is concluded that the synthesis of exopolysaccharides can be promoted effectively when feeding glucose
at a late exponential phase. |
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Keywords: | Cell dry weight Constant glucose feeding Exopolysaccharides Tremella fuciformis spores |
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