一种基于喹唑啉酮衍生物的荧光探针对次氯酸根离子的识别

次氯酸根(ClO~-)在人体免疫系统中发挥着重要的作用,其识别与检测备受关注。本文设计合成了一种含有喹唑啉酮骨架的腙型荧光探针(HEMQ),并通过~1H NMR、~(13)C NMR、高分辨质谱(HRMS)表征了其结构。探针HEMQ在V(乙醇)∶V(水)=1∶1(c(PBS)=0.02 mol/L,pH=8.7)溶液中对ClO~-具有良好的选择性且响应快速,荧光发生显著猝灭。探针HEMQ对ClO~-具有较高的灵敏度,检测限为1.0×10-4mol/L。此外,ClO~-可引起探针溶液由黄色到无色的颜色变化,因此HEMQ可作为比色、荧光双通道响应的ClO~-探针。

A Quinazolinone Derivative-based Fluorescent Probe for Hypochlorite Ion Recognition

Hypochlorite(ClO^-) displays essential roles in human immune system, the detection of which has aroused extensive attention. Herein, a hydrazone type fluorescent probe(HEMQ) that containing quinazolinone skeleton was designed and synthesized, and the structure of HEMQ was characterized by ¹H NMR, ¹³C NMR, and HRMS analysis. In V(ethanol):V(water)=1:1(c(PBS)=0.02 mol/L, pH=8.7) solution, probe HEMQ exhibits rapid response and good selectivity toward ClO^- through distinct fluorescence quenching. HEMQ displays good sensitivity to ClO^- with a detection limit of 1.0×10^-4 mol/L. In addition, ClO^- can induce a color change from yellow to colorless of the probe solution, thus, probe HEMQ can act as a colorimetric and florescent dual-channel ClO^- probe.