柘树根多糖的分离纯化及结构表征

以柘树Cudrania tricuspidata(Carr.) Bur.]的根为材料, 经热水抽提、木瓜蛋白酶-Sevag法除蛋白、乙醇沉淀和DEAE-Sephadex A-50凝胶柱层析分离纯化, 得到一种水溶性的柘树根多糖(CPS-0). 采用HPLC、糖基组成分析、甲基化分析、GC、GC-MS、NMR(1H NMR, 13C NMR及HMQC)、元素分析、UV和IR等技术对CPS-0的纯度、性质、组成和结构进行表征. 结果表明, CPS-0仅含葡萄糖, 分子量为4.6×103, 主链由1,4-连接的α-D-葡萄糖残基组成, 其侧链由末端及1,4-连接的葡萄糖残基构成, 取代于主链分支点葡萄糖的6位, 平均每10个葡萄糖残基组成的重复单元中含有1个分支.

Separation, Purification and Structure Characterization of Polysaccharide from Roots of Cudrania tricuspidata(Carr.) Bur.

A water-soluble polysaccharide(CPS-0) was obtained from the root of Cudrania tricuspidata(Carr.) Bur., by hot water extraction(70 ℃), deproteination with enzymolysis and Sevag method, precipitation with ethanol, and fractionation through DEAE-Sephadex A-50 chromatography. The purity of CPS-0 was determined via HPLC and the structure was characterized with monosaccharide composition analysis, methylation analysis, GC, GC-MS, NMR spectra(1H NMR, 13C NMR, HMQC), UV, IR, and elemental analysis. The CPS-0 was found to contain glucose residue solely, with an average repeating unit of decasaccharide, ha-ving a backbone consisting of 1,4-linked α-D-glucopyranosyl residues, to which the side chain consisting of terminal and 1,4-linked α-D-glucopyranosyl residues was attached at position 6 of the branching residues.