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A New Approach for in vitro Imaging of Breast Cancer Cells by Anti‐Metadherin Targeted PVA‐Pyrene |
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| Authors: | E. Ilker Medine Dilek Odaci Bahadir N. Gacal Burcin Gacal Serhan Sakarya Perihan Unak Suna Timur Yusuf Yagci |
| Affiliation: | 1. Division of Nuclear Chemistry, Department of Chemistry, Faculty of Science, Ege University, Bornova, 35100 Izmir, Turkey;2. Biochemistry Department, Faculty of Science, Ege University, Bornova, 35100, Izmir, Turkey;3. Department of Chemistry, Faculty of Science and Letters, Istanbul Technical University, Maslak, 34469, Istanbul, Turkey;4. Department of Infectious Diseases and Clinical Microbiology, Adnan Menderes University School of Medicine, TR‐09100 Ayd?n, Turkey |
| Abstract: | Poly(vinyl alcohol)‐pyrene‐anti‐metadherin (PVA‐Py‐(Anti‐MTDH)), a novel antibody based water soluble probe containing both fluorescent and target sites in the structure for in vitro imaging of breast cancer cells is reported here. Since breast cancer cells have an excess of MDTH protein expressed on the surface, a PVA‐Py prepared by “Click chemistry” approach is targeted by Anti‐MTDH antibody and applied to the MCF‐7 cell line. After characterization, the designed architecture was evaluated in terms of cell incorporation efficiency and compared with a non‐targeted structure (PVA‐Py). Atomic force microscopy (AFM) and fluorescence microscopy images of cells after incubation of the probe molecules were also obtained to monitor the interaction of the probes with the cancerous cells.  |
| Keywords: | drug delivery systems fluorescence functionalization of polymers imaging synthesis |