Preparative isolation of flavonoid compounds from Oroxylum indicum by high‐speed counter‐current chromatography by using ionic liquids as the modifier of two‐phase solvent system |
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Authors: | Renmin Liu Lili Xu Aifeng Li Ailing Sun |
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Institution: | College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, P. R. China |
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Abstract: | A preparative high‐speed counter‐current chromatography method for isolation and purification of flavonoid compounds from Oroxylum indicum was successfully established by using ionic liquids as the modifier of the two‐phase solvent system. Two flavonoid compounds including baicalein‐7‐O‐diglucoside and baicalein‐7‐O‐glucoside were purified from the crude extract of O. indicum by using ethyl acetate–water–C4mim]PF6] (5:5:0.2, v/v) as two‐phase solvent system. 36.4 mg of baicalein‐7‐O‐diglucoside and 60.5 mg of baicalein‐7‐O‐glucoside were obtained from 120 mg of the crude extract. Their purities were 98.7 and 99.1%, respectively, as determined by HPLC area normalization method. The chemical structures of the isolated compounds were identified by 1H‐NMR and 13C‐NMR. |
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Keywords: | Baicalein‐7‐O‐diglucoside Baicalein‐7‐O‐glucoside High‐speed counter‐current chromatography Ionic liquids Oroxylum indicum |
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