|
|||||
|
|
A fluorescence method for quantitative measurements of specific protein at powder surfaces |
|
| Authors: | Karin Landstr m Bj rn Bergenst hl Jan Alsins Mats Almgren |
| Affiliation: | aInstitute for Surface Chemistry, P.O. Box 5607, SE-114 86, Stockholm, Sweden bLund University, Department of Food Technology, Lund, Sweden cUppsala University, Department of Physical Chemistry, Uppsala, Sweden dLund University, Department of Food Engineering, Lund, Sweden |
| Abstract: | A new method using fluorescence labelled proteins was developed to determine the quantitative amount of specific protein at the powder surface. The method is based on steady-state fluorescence measurements of pyrene labelled proteins with oxygen gas phase quenching at the powder surface. The surface load of protein was measured for spray-dried dextran powders containing bovine serum albumin (BSA). The results show a patchwise surface load of about 1.3 mg m−2 at a concentration of 0.33% (dry weight) BSA in the powder. The patchwise surface load stays constant with increased BSA concentration in the powder. |
| Keywords: | Fluorescence quenching Pyrene Bovine serum albumin Spray-drying Protein adsorption |
| 本文献已被 ScienceDirect 等数据库收录！ | |