Metabolite mapping of human filarial parasite, Brugia malayi with nuclear magnetic resonance |
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Authors: | Shukla-Dave A Degaonkar M Roy R Murthy P K Murthy P S Raghunathan P Chatterjee R K |
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Institution: | * Division of Parasitology, Central Drug Research Institute, Lucknow, India ? Department of NMR, All India Institute of Medical Sciences, New Delhi, India ? Division of Medicinal Chemistry, Central Drug Research Institute, Lucknow, India § Division of Toxicology, Central Drug Research Institute, Lucknow, India |
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Abstract: | Metabolite mapping of human filarial parasite, Brugia malayi was carried out in vitro as well as in situ in host Mastomys coucha by 31P nuclear magnetic resonance (NMR) spectroscopy. Detection of parasites by visualizing contrast spots due to pathologic changes was observed by 1H magnetic resonance imaging (MRI). Major metabolites of adult B. malayi observed by 31P-NMR spectroscopy were of sugar phosphates (SP), phosphomonoesters (PME), glycerophosphoryl-ethanolamine (GPE), -choline (GPC), phosphoenolpyruvate (PEP), inorganic phosphate (Pi), nucleoside diphosphosugar and nucleotides-mono, -di and -tri phosphates. PEP and GPC were present in high concentration; PEP being the major energy reservoir and GPC the major phospholipid in this species of filaria. The 31P NMR spectra of testis of mastomys, showed seven major peaks of SP, PME, phosphocreatine (PCr), phosphodiesters (PDE), Pi, and nucleotides di- and tri-phosphates. The 31P-NMR spectra of testis of B. malayi infected animal also consisted of seven major peaks with significant decrease in the SP and PME peak showing changes in the carbohydrate and lipid metabolism of filaria infected testis. Thus, in vivo 31P MRS provided a non-invasive assessment of tissue bioenergetics and phospholipid metabolism. |
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Keywords: | 31P Nuclear Magnetic Resonance (NMR) spectroscopy 1H Magnetic Resonance Imaging (MRI) Brugia malayi Mastomys coucha Carbohydrate metabolism Lipid metabolism and filariasis |
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