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| 3-溴丙酮酸与人血清白蛋白相互作用的光谱学研究 | |
| 引用本文： | 裴明砚,郑学仿,曹洪玉,唐乾. 3-溴丙酮酸与人血清白蛋白相互作用的光谱学研究[J]. 分析化学, 2010, 38(7). DOI: 10.3724/SP.J.1096.2010.00948 |
| 作者姓名： | 裴明砚 郑学仿 曹洪玉 唐乾 |
| 作者单位： | 辽宁省生物有机化学重点实验室,大连,116622;辽宁省生物有机化学重点实验室,大连,116622;大连大学生物工程学院,大连,116622 |
| 基金项目： | 国家自然科学基金，辽宁省优秀人才培养计划项目，辽宁省高校创新团队项目，大连市科技计划项目 |
| 摘 要： | 运用荧光光谱、紫外可见吸收光谱和圆二色光谱法研究了抗肿瘤药物3-溴丙酮酸(3-Bromopyruvic acid,3-BrPA)与人血清白蛋白(Human serum albumin,HSA)的相互作用.3-BrPA对HSA的猝灭机制属于静态猝灭,并发生分子间非辐射能量转移.热力学数据显示,二者之间的作用力主要为静电作用;同步荧光光谱表明,3-BrPA与蛋白质中接近色氨酸残基的区域发生了相互作用;荧光光谱研究发现,Zn2+存在时3-BrPA对HSA的猝灭程度进一步增强;圆二色光谱法研究蛋白二级结构结果显示,3-BrPA对HSA的结构影响非常小. |
| 关 键 词： | 3-溴丙酮酸 人血清白蛋白 相互作用 荧光光谱 紫外可见光谱 圆二色光谱 |
Spectroscopic Studies on Interaction Between 3-Bromopyruvic Acid and Human Serum Albumin |
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| PEI Ming-Yan,ZHENG Xue-Fang,CAO Hong-Yu,TANG Qian. Spectroscopic Studies on Interaction Between 3-Bromopyruvic Acid and Human Serum Albumin[J]. Chinese Journal of Analytical Chemistry, 2010, 38(7). DOI: 10.3724/SP.J.1096.2010.00948 | |
| Authors: | PEI Ming-Yan ZHENG Xue-Fang CAO Hong-Yu TANG Qian |
| Affiliation: | PEI Ming-Yan1,ZHENG Xue-Fang1,2,CAO Hong-Yu1,TANG Qian1,2 1(Liaoning Key Laboratory of Bioorganic Chemistry,Dalian 116622) 2(College of Bioengineering,Dalian University,Dalian 116622) |
| Abstract: | The interaction between 3-bromopyruvic acid(3-BrPA) and human serum albumin was studied using fluorescence spectroscopy,synchronous fluorescence spectra,UV/Vis and circular dichroism spectra.Static quenching was predominant between 3-BrPA and HSA,and non-radiation energy transfer between molecules was observed.The results showed that electrostatic force played an important role in the binding reaction.Synchronous fluorescence spectral results revealed that the binding of 3-BrPA induced conformational change... |
| Keywords: | 3-Bromopyruvic acid Human serum albumin Interaction Fluorescence spectroscopy Ultraviolet spectroscopy Circular dichroism spectroscopy |
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