| 毛细管高效液相色谱-毛细管电泳二维分离接口的优化 |
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| 引用本文： | 黄爽,徐韶瑛,张祥民. 毛细管高效液相色谱-毛细管电泳二维分离接口的优化[J]. 分析化学, 2000, 28(12): 1467-1471 |
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| 作者姓名： | 黄爽 徐韶瑛 张祥民 |
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| 作者单位： | 复旦大学化学系,上海,200433;复旦大学化学系,上海,200433;复旦大学化学系,上海,200433 |
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| 基金项目： | 中国科学院资助项目,39870451, |
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| 摘 要： | 对毛细管高效液相色谱－毛细管电泳二维分离的动态脉冲接触式接口系统进行了最优化研究。讨论了它的设计原理,优化了操作条件,并对其性能进行了评价。利用这种接口,使样品在第二维的进样浓度达到第一维流出浓度的８１％,１００次连续进样峰高及迁移时间的ＲＳＤ平均值分别为３．０％、１．８％,进样时间也得以缩短,并对甘草的水提取物实现了高效二维分离。,
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| 关 键 词： | 动态接口 二维分离 毛细管高效液相色谱 毛细管电泳 |
Optimization of the Interface for Coupling Capillary High Performance Liquid Chromatography with Capillary Electrophoreis in Comprehensive Two-Dimensional System |
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| Huang Shuang,Xu Shaoying,Zhang Xiangmin. Optimization of the Interface for Coupling Capillary High Performance Liquid Chromatography with Capillary Electrophoreis in Comprehensive Two-Dimensional System[J]. Chinese Journal of Analytical Chemistry, 2000, 28(12): 1467-1471 |
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| Authors: | Huang Shuang Xu Shaoying Zhang Xiangmin |
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| Abstract: | A dynamic interface with pulse contact for coupling capillary high performance liquid chromatography with capillary electrophoreis in comprehensive two-dimensional system was developed and optimized. By this interface, injection efficiency of concentration of effluent from the first dimension into the second dimension was up to 81%. The relative standard deviations (% RSD) of peak heights and migration times of 100 consecutive runs were 3. 0% and 1. 8%, respectively. Injection time was reduced as well. Meanwhile, high efficiency separation of extracts for traditional Chinese medicine of licorice was performed in this comprehensive two-dimensional system. |
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| Keywords: | Pulse contact interface comprehensive two-dimensional system capillary high performance liquid chromatography capillary electrophoresis |
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