Exploring the interactions between gold nanoparticles and analytes through surface‐assisted laser desorption/ionization mass spectrometry |
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Authors: | Yang‐Wei Lin Wen‐Tsen Chen Huan‐Tsung Chang |
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Institution: | Department of Chemistry, National Taiwan University, Taipei, Taiwan |
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Abstract: | Surface‐assisted laser desorption/ionization mass spectrometry (SALDI‐MS) is applied to provide strong evidence for the chemical reactions of functionalized gold nanoparticles (Au NPs) with analytes – Hg2+ ions induced MPA?Au NPs aggregation in the presence of 2,6‐pyridinedicarboxylic acid (PDCA) and H2O2 induced fluorescence quenching of 11‐MUA?Au NDs. PDCA‐Hg2+‐MPA coordination is responsible for Au NPs aggregation, while the formation of 11‐MUA disulfide compounds that release into the bulk solution is responsible for H2O2‐induced fluorescence quenching. In addition to providing information about the chemical structures, SALDI‐MS is also selective and sensitive for the detection of Hg2+ ions and H2O2. The limits of detection (LODs) for Hg2+ ions and H2O2 by SALDI‐MS were 300 nM and 250 µM, respectively. The spot‐to‐spot variations in the two studies were both less than 18% (50 sample spots). Our results reveal that SALDI‐MS can be used to study analyte‐induced changes in the surface properties of nanoparticles. Copyright © 2010 John Wiley & Sons, Ltd. |
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