Two-step derivatization and mass spectral distinction of α2,3 and α2,6sialic acid linkages on N-glycans by MALDI-TOF |
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作者姓名: | Xiaoman Zhou Shang Yang Ganglong Yang Zengqi Tan Feng Guan |
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作者单位: | The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China;
; Joint International Research Laboratory of Glycobiology and Medicinal Chemistry, College of Life Science, Northwest University, Xi'an 710069, China |
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基金项目: | This study was supported by the National Natural Science Foundation of China (Nos. 81672537 and 81470294), the National Science andTechnologyMajorProjectofChina (No.2018ZX10302205) and Hundred-Talent Program of Shaanxi Province. |
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摘 要: | Sialylation is one of important glycosylation in human beings and plays an important role in cancer development. α2,3-Linked and α2,6-linked sialic acids are normally observed on the end of N-glycans and have different functions. Derivatization on sialic acid was designed to detect the different linkages by MALDI-TOF MS. In this study, a two-step derivatization by dimethylamine and ammonium hydroxide was improved to modify the sialic acid and made it easier to detect the different linkages of sialic acids on MALDI-TOF MS. Using this derivatization method, specific sialic acids linkages on N-glycans of protein samples such as fetuin and lactoferrin were detected. For complex cell samples, increased a2,3-linked and a2,6-linked sialic acids on bi-antennary and tri-antennary N-glycans were observed in A549 cells induced by hypoxia environment. Taken together, our two-step derivatization of sialic acids offers a simple and accurate way to detect specific linkages on N-glycans with MALDI-TOF mass spectrometer.
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关 键 词: | Sialic acid N-GLYCANS DERIVATIZATION MALDI-TOF HYPOXIA |
收稿时间: | 11 September 2018 |
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