| 高效毛细管电泳－电荷耦合器件检测器联用技术研究Ⅰ．多波长荧光检测装置 |
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| 引用本文： | 熊少祥,李建军,程介克.高效毛细管电泳－电荷耦合器件检测器联用技术研究Ⅰ．多波长荧光检测装置[J].色谱,1995,13(6):427-429. |
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| 作者姓名： | 熊少祥 李建军 程介克 |
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| 作者单位： | 武汉大学化学系 |
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| 基金项目： | 国家青年自然科学基金和国家教委博士点基金资助课题 |
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| 摘 要： | 报道了用电荷耦合检测器等设备组成的高效毛细管电泳多波长荧光检测装置。以荧光素和罗丹明Ｂ对其性能进行了评价，得到了具有光谱和时间分辨的三维电泳图，与被测物标准荧光光谱对照，可以直接鉴定被分离的化合物。
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| 关 键 词： | 电荷耦合器件检测器 高效毛细管电泳 罗丹明B 荧光检测 荧光素 |
Studies on the Coupling Technique between High Performance Capillary Electrophoresis and Charge-Coupled Device Detector I.A Set-up for Multiwavelength Fluorescence Detection |
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| Xiong Shaoxiang ,Li Jianjun and Cheng Jieke.Studies on the Coupling Technique between High Performance Capillary Electrophoresis and Charge-Coupled Device Detector I.A Set-up for Multiwavelength Fluorescence Detection[J].Chinese Journal of Chromatography,1995,13(6):427-429. |
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| Authors: | Xiong Shaoxiang Li Jianjun and Cheng Jieke |
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| Abstract: | new instrumental set-up combining capillary electrophoresis separation and charge-coupled device mul-tiwavelength fluorescence detection is described.The performance of the set-up was evaluated with fluores-cein and rhodamine B. A three-dimensional electrophorogram that showed the spectra and time resolutionwas obtained,on which the separated species could be identified and determined in comparing with their stan-dard fluorescence spectra. |
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| Keywords: | high performance capillary electrophoresis charge-coupled device detector fluorescence detection fluorescein rhodamine B |
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