A new C27-steroidal glycoside from Ophiopogon japonicus

A new C₂₇-steroidal glycoside with three known compounds were isolated from the tuber of Ophiopogon japonicus (Thunb.) Ker-Gawl. Based on the spectral analysis, including HRMS,¹H NMR,¹³C NMR, DEPT, ¹H-¹H COSY, HMQC and HMBC, their chemical structures were determined as pennogenin-3-O-α-α-rhamnopyranosyl-(1→2)-β-D-xyiopyranosyl-(1→4)-β-D-gluco-pyranoside (1), pennogenin-3-O-ct-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside (2), ophiopojaponin C (3) and ophiopogonin D (4).