A Highly Sensitive Method to Identify the D,L-Configurations of Monosaccharides Based on (-)-TBMB Carboxylic Acid and HPLC |
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| Authors: | Yoshihiro Nishida Chen Bai Hiroshi Ohrui Hiroshi Meguro |
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| Affiliation: | Department of Applied Biological Chemistry, Faculty of Agriculture , Tohoku University , Tsutsumidohri-Amamiyamachi 1-1, Sendai, 981, Japan |
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| Abstract: | Abstract D, L-Isomers of monosaccharides were coupled with a fluorescent chiral derivatizing agent, (-)-2-tert-butyl-2-methyl-1,3-benzodioxole-4-carboxylic acid [(-)-TBMB carboxylic acid], to afford a pair of diastereoisomers. The two were well separated with HPLC (reverse phase conditions; ODS, CH3CN:H2O:isoPrOH) within 60 min and could be detected by fluorescence of the TBMB group at the few picomolar level. |
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