A Comparative Study of Hydrodistillation and Hydrodistillation–Solvent Microextraction Methods for Identification of Volatile Components of Echinophora cinerea |
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Authors: | Hashemi Payman Abolghasemi Mir Mehdi Ghiasvand Ali Reza Ahmadi Shahla Hassanvand Hatam Yarahmadi Ali |
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Institution: | 1.Department of Chemistry, Faculty of Science, Lorestan University, Khoramabad, Iran ;2.Agriculture and Natural Resource Research Center, P.O. Box 348, Khoramabad, Iran ; |
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Abstract: | A recently developed hydrodistillation–solvent microextraction (HD–SME) method coupled to gas chromatography–mass spectrometry (GC–MS) was applied to the analysis of volatile components of aerial parts of Echinophora cinerea (Boiss). By the use of a simplex optimization method, the effects of extraction time, sample weight and microdrop volume on the extraction efficiency of the method were optimized. In the optimized conditions, 3 µL of n-heptadecane was suspended in the headspace of 6 g of hydrodistillating sample, using a microsyringe. After 7 min, the solvent was retracted back into the syringe and directly injected into the GC–MS injection port. The HD–SME method was compared to a conventional hydrodistillation technique. In general, the extraction with HD–SME was relatively faster and required smaller amounts of sample. The microextraction method also showed some selectivity towards α-phellandrene and Z-β-ocimene monoterpenes. A precision better than 6.5% (expressed as relative standard deviation) was obtained for the method. |
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