Cobalt(III), nickel(II) and ruthenium(II) complexes of 1,10-phenanthroline family of ligands: DNA binding and photocleavage studies |
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Authors: | S Arounaguiri D Easwaramoorthy A Ashokkumar Aparna Dattagupta and Bhaskar G Maiya |
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Institution: | (1) School of Chemistry, University of Hyderabad, 500 046 Hyderabad, India;(2) School of Life Sciences, University of Hyderabad, 500 046 Hyderabad, India;(3) Present address: Department of Chemistry, Crescent Engineering College, 600 048 Chennai, India |
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Abstract: | DNA binding and photocleavage characteristics of a series of mixed-ligand complexes of the type M(phen)2LL]n+ (where M = Co(III), Ni(II) or Ru(II), LL = 1,10-phenanthroline (phen), phenanthroline-dione (phen-dione) or dipyridophenazine
(dppz) andn = 3 or 2) have been investigated in detail. Various physico-chemical and biochemical techniques including UV/Visible, fluorescence
and viscometric titration, thermal denaturation, and differential pulse voltammetry have been employed to probe the details
of DNA binding by these complexes; intrinsic binding constants (K
b) have been estimated under a similar set of experimental conditions. Analysis of the results suggests that intercalative
ability of the coordinated ligands varies as dppz>phen>phen-dione in this series of complexes. While the Co(II) and Ru(II)
complexes investigated in this study effect photocleavage of the supercoiled pBR 322 DNA, the corresponding Ni(II) complexes
are found to be inactive under similar experimental conditions. Results of detailed investigations carried out inquiring into
the mechanistic aspects of DNA photocleavage by Co(phen)2(dppz)]3+ have also been reported. |
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Keywords: | Phenanthroline family of ligands metal complexes DNA binding and photocleavage |
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